FAQs – Custom Labeling

1. What is the minimum amount of antibody Columbia Biosciences can conjugate? See chart below.

DYEAMOUNT
PE
1mg
APC1mg
PerCP1mg
Dylight100-200ug
Europium100-200ug
Alexa Fluor Dyes100-200ug
Biotin100-200ug

2. What is Columbia Biosciences’ QC process?

  • Fluorescence to protein ratio, fluorometry, SEC–HPLC, antigen binding

3. How does Columbia Biosciences purify?

  • SEC-FLPC or desalting

4. What is Columbia Biosciences turnaround time?

  • Generally, one week or less

5. What ratio does Columbia Biosciences aim for when conjugating an antibody to a dye? to biotin?

  • We generally look to incorporate 1-2 biotins and 2-3 synthetic dyes per protein molecule.

6. Does Columbia Biosciences perform custom projects?

  • Yes, conjugations such as immunogens, PEG, other small molecules and bead and membranes.