Mass Spectrometry Services

Leverage our state‑of‑the‑art LC–MS platform to achieve high‑resolution intact protein analysis. Our expert team ensures optimized separations, robust mass accuracy, and reproducible data under both native and denaturing conditions, empowering precise molecular insights for your biotherapeutic and protein research.

Intact Mass Measurement Services Catalog

Catalog No.Service NameSizePrice (USD)
MIM001‑999Intact Mass Measurement by LC‑MS, Non‑Reduced1 sample$425
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MIM002‑999Intact Mass Measurement by LC‑MS, Non‑Reduced, Deglycosylated1 sample$525
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MIM003‑999Intact Mass Measurement by LC‑MS, Reduced1 sample$500
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MIM004‑999Intact Mass Measurement by LC‑MS, Reduced and Deglycosylated1 sample$600
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MIM005‑999Intact Mass Measurement by LC‑MS with DAR or F:P ratio, Non‑Reduced (Native)1 sample$475
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MIM006‑999Intact Mass Measurement by LC‑MS with DAR or F:P ratio, Non‑Reduced (Native), Deglycosylated1 sample$575
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MIM007‑999Intact Mass Measurement by LC‑MS with DAR or F:P ratio, Reduced1 sample$550
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MIM008‑999Intact Mass Measurement by LC‑MS with DAR or F:P ratio, Reduced and Deglycosylated1 sample$650
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MIM009‑999Native Intact Mass Measurement1 sample$425
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MIM010‑999Native Intact Mass Measurement, Deglycosylated1 sample$525
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MMD‑999Mass Spectrometry Method DevelopmentProject‑basedT&M
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Service Description
We utilize a high‑resolution Agilent liquid chromatography system coupled to a quadrupole time‑of‑flight (Q‑TOF) mass spectrometer to deliver precise molecular‑weight measurements of your target protein or antibody. For antibody analyses, we can optionally reduce the sample to separate light and heavy chains, then apply deconvolution algorithms to reconstruct the intact‑mass spectrum. Using these deconvoluted data, we can calculate drug‑to‑antibody ratio (DAR) and fluorophore‑to‑protein ratio (F:P), tailored to your sample type and specific requirements. If you need a native‑state “global” profile, we can skip denaturation and reduction entirely—so you get an undisturbed view of your antibody’s mass distribution.

Deliverables

  • Deconvoluted intact mass spectrum
  • Exact measured molecular weight
  • PDF report and raw data

Sample Requirements

≥ 10 µg/sample of any purified protein ≤ 180 kDa

  • Concentration & Volume
    • 1–20 μM (≈ 0.5–1 mg/mL)
    • ≥ 50 μL final volume
  • Purity & Prep
    • ≥ 90% purity (SDS‑PAGE or SEC)
    • 0.22 μm spin‑filtered to remove particulates
    • Keep samples cold (4 °C) and submit on ice

Sample Workflow

  1. Submit an online request here or visit MassSpectrometryServices.com
  2. We will email you with confirmation regarding pricing and:
    1. A Microsoft Excel spreadsheet (the Mass Spec Request (MSR) Form) for you to provide your sample information
    2. Instructions for how to send your samples
  3. Complete the MSR Form and email it to sales@columbiabiosciences.com.
  4. Send us your samples (Local Drop‑Off / Courier Service or Overnight Shipping).
    • Note: 3-hour notice required to arrange sample pickup (Courier Service)
  5. We email you a PDF report with results.

Popular Applications

  • Molecular‑weight confirmation: Verify intact antibody (or subunit) mass with high accuracy to ensure identity.
  • Protein purity assessment: Detect and quantify minor proteoforms or contaminants for a clear purity profile.
  • Precise DAR or F:P quantification: Calculate drug‑to‑antibody ratio and F:P directly from deconvoluted spectra to meet formulation specs.

LC‑MS Instrumentation Details

Our intact mass workflows run on an Agilent 1290 Infinity II UHPLC system—complete with a binary high‑pressure pump, temperature‑controlled column oven, and 25 µL loop autosampler—coupled directly to an Agilent 6545XT Q‑TOF mass spectrometer featuring Jet Stream ESI. Together, they deliver:

  • High‑Efficiency Separations: Customizable gradients on polymer, C4, C18, and SEC columns.
  • Sensitive Ionization: Jet Stream source with adjustable nebulizer and drying gas for gentle desolvation.

Additional LC‑MS Service Capabilities

MMD‑999 Mass Spec Method Development (Consultation Required)

  • Custom method optimized for your protein’s chemistry and mass range
  • Enhanced sensitivity, resolution, and reproducibility
  • Full validation: linearity, precision, robustness testing
  • Timeline: 2–4 weeks (requires 100–500 µg of sample)

Deliverables: Detailed SOPs, method transfer support, and PDF service report.