Absorbance & Emission Spectra of APC Far Red 718 from Columbia Biosciences.

SureLight® APC Far Red 718

Name: SureLight® APC Far Red 718
SKU: D67-000-10mg
Price: 300.00 USD
Availability: InStock

$300.00

SKU: D67-000-10mg Categories: APC, Far Red APC, SureLight Fluorescent Dyes (Unconjugated)

Product Details

APC Far Red 718 is a novel non-tandem allophycocyanin (APC) developed to emit in the far-red spectrum. This unique shift into the far-red region provides researchers with a brighter, more affordable, highly photostable dye that serves as an excellent alternative to Alexa Fluor 700, Spark Red 718, and APC-iFluor700 in multi-color flow cytometry panels.

Unlike tandem dyes, APC Far Red 718 avoids complications associated with energy transfer and signal decay, delivering consistent performance across experiments. Its emission profile makes it particularly valuable for applications requiring reduced spectral overlap and precise data resolution. Third-party researchers have reported that APC Far Red 718 offers 40% better performance when compared to APC-iFluor 700.

We are now offering APC Far Red 718 in native dye format (10mg size), enabling researchers to label their own antibodies for use in flow cytometry or other fluorescence-based applications. With its ease of labeling and compatibility across standard flow cytometers (633nm/red laser), this dye empowers scientists to design customized experiments with confidence and flexibility. Use of APC Far Red 718 requires no licensing or royalties. APC Far Red 718 is for research use only, not for diagnostic or therapeutic use.

Columbia Biosciences offers custom conjugation and labeling services leveraging APC Far Red 718, click here to learn more about our custom conjugation services.

Please feel free to contact us if you have any questions about APC Far Red 718.

Use:

Custom Antibody Labeling Flow Cytometry/FACS

Size:

10mg

Concentration:

10mg/ml

Excitation max. λ:

713nm

Emission max. λ:

718nm

Form / Storage:

Supplied as lyophilized powder. Upon receipt, store at 2-8°C.

Stability:

Product should be stored at 2–8°C in the dark. Product is stable for up to 1 year upon resuspension.

Technical Info:

Spectral Characteristics

Absorbance maximum: 708 nm ± 2 nm (additional absorption peak at 614 ± 2 nm)
Excitation maximum: 713 nm ± 2 nm
Emission maximum: 718 nm ± 2 nm
Au/mg: 5.3
Extinction Coefficient (ε): 6.12 x 10⁵ M^-1cm^-1
Quantum Yield (QY): 0.60
Brightness (ε x QY): 3.67 x 10⁵ M^-1 cm-1

Specifications

A708/A280: ≥ 4.0
HPLC-SEC: ≥ 95%

Absorbance, excitation, and emission measured in 100mM NaPO_{4 ,}100mM NaCl pH7.4 buffer. The emission scan was taken with excitation at 612 nm. The excitation scan was taken with emission at 740 nm.

Check out the spectra for APC Far-Red 718 and our results from an in-house Flow Cytometry analysis comparison of CD19 expression on human B-lymphocytes (Raji, Burkitt’s Lymphoma) below.

Absorbance & Emission Spectra of APC Far Red 718 from Columbia Biosciences.