Detection of mouse IgG using Columbia Biosciences anti-mouse IgG conjugated to DyLight? 488:

Raji B cells were stained for 30 minutes with 5 ?g/ml Columbia Biosciences anti-mouse IgG conjugated to DyLight 488 after treatment for 1 hour with staining buffer alone (white histogram), staining buffer containing mouse anti-human CD45 primary antibody (5 ?g/ml; black histogram), or staining buffer containing mouse IgG isotype control (red-outlined histogram). 10,000 cells per sample were analyzed on a flow cytometer for fluorescence emission at 530 nm.

Flow cytometry analysis of CD45 expression on Raji (CCL-86, ATCC) human B cell lymphoma cell line.

The cells were stained with 1 ug/ml of either mouse anti human CD45 antibodies ? shaded histogram; or with isotype matched control ? open histogram. Both samples were washed and stained with 0.5 ug/ml of goat anti-Mouse IgG DyLight? 488 conjugate. The stained cells were analyzed on BD FACSCalibur instrument.