Detection of rabbit IgG using Columbia Biosciences anti-rabbit IgG conjugated to DyLight? 488: Raji B cells were stained for 30 minutes with 5 ?g/ml Columbia Biosciences anti-rabbit IgG conjugated to DyLight? 488 after treatment for 1 hour with staining buffer alone (white histogram), staining buffer containing rabbit anti-human CD45 primary antibody (1 ?g/ml; black histogram), or staining buffer containing rabbit IgG isotype control (red-outlined histogram). 10,000 cells per sample were analyzed on a flow cytometer for fluorescence emission at 530 nm.