Goat Anti-Rabbit IgG: SureLight® R-PE;1mg
Lyophilized material is stable for up to one year.? After product has been reconstituted, product should be stored at 2-8?C in the dark and be used within 3 months. ?If further dilution of the conjugate is required, use diluted material within one week.
Detection of rabbit IgG using Columbia Biosciences anti-rabbit IgG conjugated to R-PE: Raji B cells were stained for 30 minutes with 5 ?g/ml Columbia Biosciences anti-mouse IgG conjugated to R-phycoerythrin after treatment for 1 hour with staining buffer alone (white histogram), staining buffer containing rabbit anti-human CD45 primary antibody (1 ?g/ml; black histogram), or staining buffer containing rabbit IgG isotype control (red-outlined histogram). 10,000 cells per sample were analyzed on a flow cytometer for fluorescence emission at 585 nm.
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