Monoclonal Anti-Poly Histidine Antibody

$175.00

SKU: FITC-1711 Category:

Description

Monoclonal Anti-poly Histidine Antibody, 100ug

Clone Number: AD1.1.10

Conjugate: FITC

Preparation: This antibody was produced from cell culture elicited from a mouse immunized with a polypeptide containing polyhistidine tag. The cell culture was purified on Protein G and the resulting antibody pool was then polished by size exclusion chromatography (Superdex-200) to remove aggregated IgG.

Specificity: polypeptide containing a polyhistidine tag

Immunogen: polyhistidine tagged polypeptide (PAX6 transcription factor linked to histidine tag)

IgG class: mouse IgG1

Applications: Flow Cytometry (1/10 dilution), Immunofluorescence (assay dependent), Western Blot (1:250 dilution).

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Antibody:
Size:
100ug
Concentration:
0.1 mg/mL
Dye:
FITC
Specificity:
This antibody has been selected for its ability to detect proteins containing accessible consecutive histidine regions. The antibody has been shown to detect polyhistidine tags localized at both the amino- or carboxyl-terminus.
Form / Storage:
Supplied as a 0.2 µm filtered solution in PBS, pH 7.4 with 1% BSA. Can be stored at 2-8ºC.