Mouse Anti-6His IgG: Eu-W1024

$380.00$3,183.00

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SKU: D17-1711 Category:

Product Details

Clone: AD1.1.10

Antibody:
Size:
20ug, 50ug, 1mg
Concentration:
1mg/ml
Excitation max. λ:
340nm
Emission max. λ:
615nm
Conjugate/Tag:
Europium Chelate W-1024
Form / Storage:
Conjugate supplied as a liquid in 50 mM Tris (pH 7.8) + 0.9% NaCl + 0.05% NaN3. Upon receipt, store at 2-8°C in the dark. DO NOT FREEZE.
Stability:

Product should be stored at 2-8°C in the dark and be used within 6 months.

Technical Info:

 

 

 

 

 

 

 

FRET assay detecting multiple epitope-tagged protein. Dilutions of a recombinant protein containing multiple different epitope tags were incubated with antibodies labeled with either a europium chelate (donor) or DyLight®650 (acceptor). The assay was performed in a white 384 well microplate with a total volume per well of 20 µl, with all reagent dilutions being made in 25 mM Tris, pH 7.5, 100 mM NaCl, 0.1% BSA, and 0.05% Tween-20. Europium labeled donor antibody was at a final concentration in well of 2 nM, while the DyLight®650 labeled acceptor antibody was at 25 nM. Plates were read after 1 hour, measuring energy transfer signal at 665nm using a Perkin Elmer Victor3V time-resolved multilabel reader.

 

References:

  • Coward, P., Conn, M., Tang, J., Xiong, F., Menjares, A., & Reagan, J. D. (2009). Application of an allosteric model to describe the interactions among retinol binding protein 4, transthyretin, and small molecule retinol binding protein 4 ligands. Anal Biochem (Vol. 384).
  • Day, J. E. H., Sharp, S. Y., Rowlands, M. G., Aherne, W., Workman, P., & Moody, C. J. (2010). Targeting the Hsp90 chaperone: synthesis of novel resorcylic acid macrolactone inhibitors of Hsp90. Chemistry (Vol. 16).
  • Vassilev, L. T., Tovar, C., Chen, S., Knezevic, D., Zhao, X., Sun, H., et al. (2006). Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. PNAS (Vol. 103).
  • Zhou, V., Han, S., Brinker, A., Klock, H., Caldwell, J., & Gu, X. -ju. (2004). A time-resolved fluorescence resonance energy transfer-based HTS assay and a surface plasmon resonance-based binding assay for heat shock protein 90 inhibitors. Anal Biochem (Vol. 331).

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