Flow Cytometry analysis of transfected HEK293 cells. Fixed and permeabilized cells stained with 1 µg/ml of Mouse anti-6His IgG: SureLight APC. ​

Shaded histogram – HEK293 cells, expressing 6His-tagged protein.​

Open histogram – mock transfected control HEK293 cells.

References

• Coward, P., Conn, M., Tang, J., Xiong, F., Menjares, A., & Reagan, J. D. (2009). Application of an allosteric model to describe the interactions among retinol binding protein 4, transthyretin, and small molecule retinol binding protein 4 ligands. Anal Biochem (Vol. 384).
• Day, J. E. H., Sharp, S. Y., Rowlands, M. G., Aherne, W., Workman, P., & Moody, C. J. (2010). Targeting the Hsp90 chaperone: synthesis of novel resorcylic acid macrolactone inhibitors of Hsp90. Chemistry (Vol. 16).
• Vassilev, L. T., Tovar, C., Chen, S., Knezevic, D., Zhao, X., Sun, H., et al. (2006). Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. PNAS (Vol. 103).
• Zhou, V., Han, S., Brinker, A., Klock, H., Caldwell, J., & Gu, X. -ju. (2004). A time-resolved fluorescence resonance energy transfer-based HTS assay and a surface plasmon resonance-based binding assay for heat shock protein 90 inhibitors. Anal Biochem (Vol. 331).

TR-FRET ResourcesLearn more about how TR-FRET assays work

Additional TR-FRET Assay Reagents Explore additional reagents for enhanced assay performance