Rabbit anti-V5 IgG: Eu-1024
$419.00 – $3,509.00
Product should be stored at 2-8?C in the dark and be used within 6 months.
FRET assay detecting multiple epitope-tagged protein. Dilutions of a recombinant protein containing multiple different epitope tags were incubated with antibodies labeled with either a europium chelate (donor) or DyLight?650 (acceptor). The assay was performed in a white 384 well microplate with a total volume per well of 20 ?l, with all reagent dilutions being made in 25 mM Tris, pH 7.5, 100 mM NaCl, 0.1% BSA, and 0.05% Tween-20. Europium labeled donor antibody was at a final concentration in well of 2 nM, while the DyLight?650 labeled acceptor antibody was at 25 nM. Plates were read after 1 hour, measuring energy transfer signal at 665nm using a Perkin Elmer Victor3V time-resolved multilabel reader.
- Coward, P., Conn, M., Tang, J., Xiong, F., Menjares, A., & Reagan, J. D. (2009). Application of an allosteric model to describe the interactions among retinol binding protein 4, transthyretin, and small molecule retinol binding protein 4 ligands. Anal Biochem (Vol. 384).
- Day, J. E. H., Sharp, S. Y., Rowlands, M. G., Aherne, W., Workman, P., & Moody, C. J. (2010). Targeting the Hsp90 chaperone: synthesis of novel resorcylic acid macrolactone inhibitors of Hsp90. Chemistry (Vol. 16).
- Vassilev, L. T., Tovar, C., Chen, S., Knezevic, D., Zhao, X., Sun, H., et al. (2006). Selective small-molecule inhibitor reveals critical mitotic functions of human CDK1. PNAS (Vol. 103).
- Zhou, V., Han, S., Brinker, A., Klock, H., Caldwell, J., & Gu, X. -ju. (2004). A time-resolved fluorescence resonance energy transfer-based HTS assay and a surface plasmon resonance-based binding assay for heat shock protein 90 inhibitors. Anal Biochem (Vol. 331).