SureLight™ Allophycocyanin (APC), 1mg

$53.00

SKU: D3-000 Category:

Description

Crosslinked Allophycocyanin, 1 mg

Please contact us for bulk pricing.
Isolated allophycocyanin dissociates when in diluted solutions or when it is exposed to chaotropic salts, therefore APC is usually cross-linked such that it is stable for use in biological assay formats. Columbia Biosciences uses a proprietary, non-destructive cross-linking process that increases the thermal stability while maintaining fluorescent and spectral integrity of the native dye.
Structural Characteristics
Allophycocyanin can be isolated from various species of red or blue-green algae, each producing slightly different forms of the molecule. It is composed of two different subunits (α and β) in which each subunit has one phycocyanobilin (PCB) chromophore. The subunit structure for APC has been determined as (αβ)3. The molecular weight of APC is 105,000 Daltons.
Spectral Characteristics

Absorption maximum
652 nm

Additional absorption peak
625 nm

Emission maximum
657.5 nm

Extinction Coefficient (ε)
2.4 x 105 M-1cm-1

Quantum Yield (QY)
0.68

Brightness (ε x QY)
1.6 x 105 M-1cm-1

Specifications

A650/A280 ≥ 4.5

A650/A620 ≥ 1.25

Cross-link Ratio ≥ 1.0

Cross-link ratio is a measure of A650/A620 by diluting 0.1 mg SureLight® APC into 1 ml 50 mM Tris-HCl, 1 M sodium perchlorate, pH 7.0

Download Info Sheet Technical Data Sheet
MSDS
 
Lot Specific Tech Sheets
APC000-15-035
APC000-15-036
APC000-16-037

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Crosslinked Allophycocyanin, 1 mg

Please contact us for bulk pricing.
Isolated allophycocyanin dissociates when in diluted solutions or when it is exposed to chaotropic salts, therefore APC is usually cross-linked such that it is stable for use in biological assay formats. Columbia Biosciences uses a proprietary, non-destructive cross-linking process that increases the thermal stability while maintaining fluorescent and spectral integrity of the native dye.
Structural Characteristics
Allophycocyanin can be isolated from various species of red or blue-green algae, each producing slightly different forms of the molecule. It is composed of two different subunits (α and β) in which each subunit has one phycocyanobilin (PCB) chromophore. The subunit structure for APC has been determined as (αβ)3. The molecular weight of APC is 105,000 Daltons.
Spectral Characteristics

Absorption maximum
652 nm

Additional absorption peak
625 nm

Emission maximum
657.5 nm

Extinction Coefficient (ε)
2.4 x 105 M-1cm-1

Quantum Yield (QY)
0.68

Brightness (ε x QY)
1.6 x 105 M-1cm-1

Specifications

A650/A280 ≥ 4.5

A650/A620 ≥ 1.25

Cross-link Ratio ≥ 1.0

Cross-link ratio is a measure of A650/A620 by diluting 0.1 mg SureLight® APC into 1 ml 50 mM Tris-HCl, 1 M sodium perchlorate, pH 7.0

Download Info Sheet Technical Data Sheet
MSDS
 
Lot Specific Tech Sheets
APC000-15-035
APC000-15-036
APC000-16-037