SureLight® B-Phycoerythrin ( B-PE)
$108.00 – $5,410.00
Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.
It is compatible with commonly available lasers and gives exceptional results in flow cytometry, Luminex® and immunofluorescent staining. B-PE is also less “sticky” than common synthetic fluorophores and therefore gives less background interference.
Phycobiliproteins are sensitive to freeze-thaw cycles. Avoid exposure to heat and light.
B-phycoerythrin is produced by red algae such as Rhodella sp. The protein is made up of at least three different subunits and varies according to the species of algae that produces it. The subunit structure of the most common B-PE is (αβ)6γ. The α subunit has two phycoerythrobilins (PEB), the β subunit has 3 PEBs and the γ subunit has 2 PEB and 2 phycourobilins (PUB). The molecular weight of B-PE is 240,000 Daltons.
Excitation and emission profiles for SureLight™ B-PE. (Emission scan excitation wavelength at 540 nm.)
- 532 nm – Diode-Pumped Solid State (DPSS) Laser
- 543 nm – He/Ne Laser
- Absorption maximum -545 nm
- Additional Absorption peak – 563.5
- Emission maximum -572 nm
- Extinction Coefficient (ε) – 2.41 x 106 M-1 cm-1
- Quantum Yield (QY) – 0.98
- Brightness (ε x QY) -2.36 x 106 M-1 cm-1
- A545/A280 ≥ 5.5
- A620/A545 < 0.01
- >95% single peak by HPLC
SureLight™ B-Phycoerythrin ( B-PE) Labeling Protocol:
REF: M. Roederer, Conjugation of monoclonal antibodies (August, 2004). http://www.drmr.com/abcon/