$156.00 – $10,404.00
Many applications and instruments were developed specifically for PE-777 (PE Cyanine 7.0). It is commonly used in immunoassays such as FACS, flow cytometry, multimer/tetramer applications. With new instrumentation available, PE-777 is also well suited for immunohistochemistry (IHC).
SureLight™ PE-777 (PE Cyanine 7.0) Labeling Protocol:
REFS: M Roederer, AB Kantor, DR Parks, and LA Herzenberg: Cy7PE and Cy7APC: Bright new probes for immunofluorescence. Cytometry, 24:191-197 (1996).
Hardy, RR: Purification and coupling of fluorescent proteins for use in flow cytometry. In: Handbook of Experimental Immunology, 4th ed. DM Weir, LA Herzenberg, C Blackwell, and LA Herzenberg, editors. Blackwell Scientific Publications, Boston, 1986, pp. 31.1-31.12.
R-phycoerythrin is predominantly produced by red algae. The protein is made up of at least three different subunits and varies according to the species of algae that produces it. The subunit structure of the most common R-PE is (αβ)6γ. The α subunit has two phycoerythrobilins (PEB), the β subunit has 2 or 3 PEBs and one phycourobilin (PUB), while the different gamma subunits are reported to have 3 PEB and 2 PUB (γ1) or 1 or 2 PEB and 1 PUB (γ2). The molecular weight of R-PE is 250,000 Daltons. PE-777 is a tandem fluorophore comprising R-Phycoerythin and a red-shifted cyanine dye.