SureLight™ R-Phycoerythrin (R-PE), 1mg

$10.00

SKU: D5-000 Category:

Description

SureLight™ R-Phycoerythrin in 60% Ammonium Sulfate, 1mg. Please contact us for bulk pricing.

Applications for R-Phycoerythrin
Many applications and instruments were developed specifically for R-phycoerythrin. It is commonly used in immunoassays such as FACS, flow cytometry, multimer/tetramer applications. With new instrumentation available, R-PE is also well suited for immunohistochemistry (IHC) and Western blot applications.

Reviews

There are no reviews yet.

Be the first to review “SureLight™ R-Phycoerythrin (R-PE), 1mg”

Your email address will not be published. Required fields are marked *

SureLight™ R-Phycoerythrin in 60% Ammonium Sulfate, 1mg. Please contact us for bulk pricing.

Applications for R-Phycoerythrin
Many applications and instruments were developed specifically for R-phycoerythrin. It is commonly used in immunoassays such as FACS, flow cytometry, multimer/tetramer applications. With new instrumentation available, R-PE is also well suited for immunohistochemistry (IHC) and Western blot applications.

Technical Info:

Structural Characteristics
R-phycoerythrin is predominantly produced by red algae. The protein is made up of at least three different subunits and varies according to the species of algae that produces it. The subunit structure of the most common R-PE is (αβ)6γ. The α subunit has two phycoerythrobilins (PEB), the β subunit has 2 or 3 PEBs and one phycourobilin (PUB), while the different gamma subunits are reported to have 3 PEB and 2 PUB (γ1) or 1 or 2 PEB and 1 PUB (γ2). The molecular weight of R-PE is 250,000 Daltons.

Excitation and emission profiles for SureLight™ R-PE versus alternate source R-PE at a standard protein concentration. (Emission scan excitation wavelength at 488 nm.)
Comparative emission levels of SureLight™ R-PE versus alternate source R-PE at common instrument laser excitation wavelengths at a standard protein concentration.

Excitation wavelengths correspond to :

  • 488 nm – Argon Laser
  • 532 nm – Diode-Pumped Solid State (DPSS) Laser
  • 543 nm – He/Ne Laser

Spectral Characteristics

  • Absorption maximum – 565 nm
  • Additional Absorption peak – 498 nm
  • Emission maximum – 573 nm
  • Extinction Coefficient (ε) -1.96 x 106 M-1cm-1
  • Quantum Yield (QY) – 0.84
  • Brightness (ε x QY)- 1.65 x 106 M-1cm-1

Specifications

  • A566/A280 ≥ 5.0
  • A566/A498 < 1.5
  • A620/A566 < 0.01
  • Functional activity Relative QY > 2.50
  • >95% single peak by HPLC

Lot-specific Technical Data Sheets

  • RPE000-14-022
  • PE000-14-023
  • RPE000-14-024