Both Western Blot and ELISA measure the immune system’s response to an infectious agent rather than looking for the components of the agent itself.
Western Blot is an antibody detection procedure where a mixture of proteins is separated based on molecular weight through gel electrophoresis. These results are then transferred to a membrane producing a band for each protein. The membrane is then incubated with labeled antibodies specific to the protein of interest.
In an ELISA, an antigen must be immobilized on a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate to produce a measureable product. The most crucial element of the detection strategy is a highly specific antibody-antigen interaction.
Columbia Biosciences manufactures and sells both secondary antibody conjugates and streptavidin conjugates for use in both Western Blot and ELISA.
Showing 1–10 of 29 results
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Goat Anti-GST IgG: Alkaline Phosphatase, 100ug
Goat anti-GST (Glutathione-S-Transferase) IgG conjugated to Alkaline Phosphatase, 100ug Antibody: Goat polyclonal...
$350.00#AP-1310Add to cart Details -
Goat anti-Human IgG Fc-specific: HRP, 1mg
Goat anti-Human IgG, Fc-specific conjugated to HRP, 1mg Antibody: Goat polyclonal anti-Human...
$158.00#HRP-110-FcAdd to cart Details -
Goat anti-Human IgG, H+L: HRP, 1mg
Goat anti-Human IgG, H+L conjugated to HRP, 1mg Antibody: Goat polyclonal anti-Human...
$158.00#HRP-110Add to cart Details -
Goat Anti-Mouse IgG Fc-spec: HRP, 1mg
Goat Anti-Mouse IgG, Fc-specific conjugated to HRP, 1mg Antibody: Goat polyclonal...
$158.00#HRP-112-FcAdd to cart Details -
Goat Anti-Mouse IgG, H+L: HRP, 1mg
Goat Anti-Mouse IgG, H+L conjugated to HRP, 1mg Antibody: Goat polyclonal anti-Mouse...
$158.00#HRP-112Add to cart Details -
Goat Anti-Mouse IgG: Alkaline Phosphatase, 100ug
Goat Anti-Mouse IgG conjugated to Alkaline Phosphatase, 100ug Antibody: Goat polyclonal anti-Mouse...
$158.00#AP-112Add to cart Details -
Goat Anti-Rabbit IgG, H+L: HRP, 1mg
Goat Anti-Rabbit IgG, H+L conjugated to HRP, 1mg Antibody: Goat polyclonal...
$158.00#HRP-114Add to cart Details -
Goat anti-Rabbit IgG: Alkaline Phosphatase, 100ug
Goat anti-Rabbit IgG conjugated to Alkaline Phosphatase, 100ug Antibody: Goat polyclonal anti-Rabbit...
$158.00#AP-114Add to cart Details -
Monoclonal Anti-Poly Histidine Antibody
Monoclonal Anti-poly Histidine Antibody, 100ug Clone Number: AD1.1.10 Conjugate: FITC Preparation:...
$179.00#FITC-1711Add to cart Details -
Mouse Anti-6His IgG: Alkaline Phosphatase, 100ug
Mouse Anti-6His (6x Histidine) IgG (Clone AD1.1.10) conjugated to Alkaline Phosphatase,...
$350.00#AP-1711Add to cart Details