Both Western Blot and ELISA measure the immune system’s response to an infectious agent rather than looking for the components of the agent itself.
Western Blot is an antibody detection procedure where a mixture of proteins is separated based on molecular weight through gel electrophoresis. These results are then transferred to a membrane producing a band for each protein. The membrane is then incubated with labeled antibodies specific to the protein of interest.
In an ELISA, an antigen must be immobilized on a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate to produce a measureable product. The most crucial element of the detection strategy is a highly specific antibody-antigen interaction.
Columbia Biosciences manufactures and sells both secondary antibody conjugates and streptavidin conjugates for use in both Western Blot and ELISA.
Showing 11–20 of 29 results
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Mouse Anti-6His IgG: HRP, 100ug
Horseradish Peroxidase conjugated to Mouse Anti-6 Histidine, 100ug Antibody: Mouse monoclonal...
$350.00#HRP-1711Add to cart Details -
Mouse Anti-6His IgG: unconjugated 100ug
Mouse Anti-6His IgG, unconjugated, 100ug Antibody: Mouse anti-6x Histidine IgG Clone:...
$350.00#00-1711-100Add to cart Details -
Mouse Anti-6His IgG: unconjugated 1mg
Mouse Anti-6His IgG, unconjugated, 1 mg Antibody: Mouse anti-6x Histidine IgG Clone:...
$669.00#00-1711-1MGAdd to cart Details -
Mouse Anti-6xHistidine IgG: Biotin 100ug
Mouse Anti-6xHistidine IgG1 conjugated to Biotin, 100ug Antibody: Mouse monoclonal anti-6xHistidine...
$350.00#B1-1711Add to cart Details -
Mouse Anti-C-MYC IgG: Alkaline Phosphatase, 100ug
Mouse Anti-C-MYC IgG clone 9E10 (EQKLISEEDL) conjugated to Alkaline Phosphatase, 100ug...
$350.00#AP-1714Add to cart Details -
Mouse Anti-C-MYC IgG: Biotin, 100ug
Mouse Anti-C-MYC IgG clone 9E10 (EQKLISEEDL) conjugated to Biotin, 100ug Antibody:...
$350.00#B1-1714Add to cart Details -
Mouse Anti-DYKDDDDK (clone M2) IgG: HRP, 100ug
Horseradish Peroxidase conjugated to Mouse Anti-DYKDDDDK (clone M2) Clone: M2 Enzyme:...
$350.00#HRP-1718Add to cart Details -
Mouse anti-DYKDDDDK IgG: Alkaline Phosphatase, 100ug
Mouse anti-DYKDDDDK IgG (Clone M2) conjugated to Alkaline Phosphatase, 100ug Antibody: Mouse...
$350.00#AP-1718Add to cart Details -
Mouse Anti-HA IgG: Alkaline Phosphatase, 100ug
Mouse Anti-HA (Hemagglutinin) IgG1 (Clone 16B12) conjugated to Alkaline Phosphatase, 100ug...
$350.00#AP-1722Add to cart Details -
Mouse Anti-HA IgG: Biotin, 100ug
Mouse Anti-HA (Hemagglutinin) IgG1 (Clone 16B12) conjugated to Biotin, 100ug Antibody:...
$350.00#B1-1722Add to cart Details