Both Western Blot and ELISA measure the immune system’s response to an infectious agent rather than looking for the components of the agent itself.
Western Blot is an antibody detection procedure where a mixture of proteins is separated based on molecular weight through gel electrophoresis. These results are then transferred to a membrane producing a band for each protein. The membrane is then incubated with labeled antibodies specific to the protein of interest.
In an ELISA, an antigen must be immobilized on a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate to produce a measureable product. The most crucial element of the detection strategy is a highly specific antibody-antigen interaction.
Columbia Biosciences manufactures and sells both secondary antibody conjugates and streptavidin conjugates for use in both Western Blot and ELISA.
Showing 21–29 of 29 results
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Mouse Anti-HA IgG: HRP, 100ug
Mouse Anti-HA (Hemagglutinin) IgG1 (Clone 16B12) HRP, 100ug Antibody: Mouse monoclonal...
$350.00#HRP-1722Add to cart Details -
Rabbit Anti-S tag IgG: Alkaline Phosphatase, 100ug
Rabbit anti-S tag IgG conjugated to Alkaline Phosphatase, 100ug Antibody: Rabbit polyclonal...
$350.00#AP-1831Add to cart Details -
Rabbit Anti-S tag IgG: Biotin, 100ug
Rabbit Anti-S tag IgG conjugated to Biotin, 100ug Antibody: Rabbit polyclonal anti-S-tag...
$350.00#B1-1831Add to cart Details -
Rabbit Anti-T7 IgG: Alkaline Phosphatase, 100ug
Rabbit Anti-T7 IgG conjugated to Alkaline Phosphatase, 100ug Antibody: Rabbit polyclonal...
$350.00#AP-1832Add to cart Details -
Rabbit Anti-T7 IgG: Biotin, 100ug
Rabbit Anti-T7 IgG conjugated to Biotin, 100ug Antibody: Rabbit polyclonal anti-T7...
$350.00#B1-1832Add to cart Details -
Rabbit Anti-V5 IgG: Alkaline Phosphatase, 100ug
Antibody: Rabbit polyclonal Anti-V5 (GKPIPNPLLGLDST) IgG Enzyme: Alkaline Phosphatase Uses: Western blot detection...
$350.00#AP-1834Add to cart Details -
Rabbit Anti-V5 IgG: Biotin, 100ug
Rabbit Anti-V5 IgG conjugated to Biotin, 100ug Antibody: Rabbit polyclonal anti-V5...
$350.00#B1-1834Add to cart Details -
Streptavidin: Alkaline Phosphatase, 100ug
Streptavidin conjugated to Alkaline Phosphatase (AP), 100ug Binding Moiety: Streptavidin Enzyme: Alkaline Phosphatase...
$115.00#AP-2212Add to cart Details -
Streptavidin: HRP, 1mg
Binding Moiety: Streptavidin Enzyme:Horseradish Peroxidase Uses: Western blot detection. Recommended dilution of...
$168.00#HRP-2212-1MGAdd to cart Details