Both Western Blot and ELISA measure the immune system’s response to an infectious agent rather than looking for the components of the agent itself.
Western Blot is an antibody detection procedure where a mixture of proteins is separated based on molecular weight through gel electrophoresis. These results are then transferred to a membrane producing a band for each protein. The membrane is then incubated with labeled antibodies specific to the protein of interest.
In an ELISA, an antigen must be immobilized on a solid surface and then complexed with an antibody that is linked to an enzyme. Detection is accomplished by assessing the conjugated enzyme activity via incubation with a substrate to produce a measureable product. The most crucial element of the detection strategy is a highly specific antibody-antigen interaction.
Columbia Biosciences manufactures and sells both secondary antibody conjugates and streptavidin conjugates for use in both Western Blot and ELISA.
Showing 21–30 of 39 results
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Mouse Anti-C-MYC IgG: Alkaline Phosphatase;100ug
Clone: 9E10 (EQKLISEEDL) Reactivity: c-myc tagged proteins Immunogen: 9E10 epitope (EQKLISEEDL)...
$386.00#AP-1714Add to cart Details -
Mouse Anti-C-MYC IgG: Biotin;100ug
Clone: 9E10 (EQKLISEEDL) Reactivity: c-myc tagged proteins Immunogen: 9E10 epitope (EQKLISEEDL...
$386.00#B1-1714Add to cart Details -
Mouse Anti-DYKDDDDK (clone M2) IgG: HRP;100ug
Clone: M2 Recommended dilution of 1:500 – 1:10000. Starting dilution: 1:2000.
$386.00#HRP-1718Add to cart Details -
Mouse anti-DYKDDDDK IgG: Alkaline Phosphatase;100ug
Clone: M2 Recommended dilution of 1:500 – 1:10000. Starting dilution: 1:2000.
$386.00#AP-1718Add to cart Details -
Mouse Anti-HA IgG: Alkaline Phosphatase;100ug
Clone: 16B12 Reactivity: Human influenza hemagglutinin (HA) tagged proteins Immunogen: twelve...
$386.00#AP-1722Add to cart Details -
Mouse Anti-HA IgG: Biotin;100ug
Clone: 16B12 Reactivity: Human influenza hemagglutinin (HA) tagged proteins Immunogen: twelve...
$386.00#B1-1722Add to cart Details -
Mouse Anti-HA IgG: HRP;100ug
Clone: 16B12 Recommended dilution of 1:500 – 1:10000. Starting dilution: 1:2000.
$386.00#HRP-1722Add to cart Details -
Rabbit Anti-S tag IgG: Alkaline Phosphatase;100ug
Reactivity: S-tagged proteins Immunogen: KETAAAKFERQHMDS Recommended dilution of 1:500 – 1:10000....
$386.00#AP-1831Add to cart Details -
Rabbit Anti-S tag IgG: Biotin;100ug
Reactivity: S-tagged proteins Immunogen: KETAAAKFERQHMDS Recommended dilution of 1:500 – 1:10000....
$386.00#B1-1831Add to cart Details -
Rabbit Anti-T7 IgG: Alkaline Phosphatase;100ug
Reactivity: T7-tagged proteins Immunogen: T7 (MASMTGGGQQMG) Recommended dilution of 1:500 –...
$386.00#AP-1832Add to cart Details